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Anti-Calnexin antibody - ER Membrane Marker (ab75801)

Price and availability

335 040 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Calnexin antibody - ER Membrane Marker (ab75801)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Calnexin - ER Membrane Marker
  • Suitable for: ICC/IF, IHC-P, IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Calnexin antibody - ER Membrane Marker
    See all Calnexin primary antibodies
  • Description

    Rabbit polyclonal to Calnexin - ER Membrane Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Dog Calnexin (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • General notes

    References regarding specificity

    Horner SM  et al. Mitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus. Proc Natl Acad Sci U S A 108:14590-5 (2011). PubMed: 21844353

    Myhill N  et al. The subcellular distribution of calnexin is mediated by PACS-2. Mol Biol Cell 19:2777-88 (2008). PubMed: 18417615

    Yoshimura SI  et al. Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus. J Cell Sci 114:4105-15 (2001). PubMed: 11739642

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Neuroscience
    • Sensory System
    • Visual system

Images

  • Western blot - Anti-Calnexin antibody - ER Membrane Marker (ab75801)
    Western blot - Anti-Calnexin antibody - ER Membrane Marker (ab75801)
    Anti-Calnexin antibody - ER Membrane Marker (ab75801) at 1/2000 dilution ((in TBS with 0.05% TW-20)) + HeLa cell lysate

    Secondary
    An HRP-conjugated goat anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - ER Membrane Marker (ab75801)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - ER Membrane Marker (ab75801)

    ab75801 staining Calnexin in juvenile mouse backskin tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunoprecipitation - Anti-Calnexin antibody - ER Membrane Marker (ab75801)
    Immunoprecipitation - Anti-Calnexin antibody - ER Membrane Marker (ab75801)

    ab75801 used in Immunoprecipitation of Calnexin from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate.

    20µl of protein G beads were used in Immunoprecipitation.  

  • Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Membrane Marker (ab75801)
    Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Membrane Marker (ab75801)
    ab75801 staining Calnexin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rabbit IgG secondary antibody was used.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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