Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µg 274 732 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Rabbit polyclonal to Calbindin
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human
Isotype: IgG

Product name

Anti-Calbindin antibody
See all Calbindin primary antibodies
Description

Rabbit polyclonal to Calbindin
Host species

Rabbit
Specificity

We recommend the use of RabMAb ab108404 for testing in IHC and ICC/IF for this target.
Tested applications

Suitable for: IP, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Hamster, Cow, Pig
Immunogen

Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Calbindin.

Read Abcam's proprietary immunogen policy (Peptide available as ab60452.)

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Recombinant Protein
- Recombinant Human Calbindin protein (ab82631)

Our Abpromise guarantee covers the use of ab49899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		Use at an assay dependent concentration.
WB		Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 30 kDa).

Function

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Sequence similarities

Belongs to the calbindin family.
Contains 5 EF-hand domains.
Domain

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
Target information above from: UniProt accession P05937 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 539930 Cow
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 100153645 Pig
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P04467 Cow
- SwissProt: P05937 Human
- SwissProt: P12658 Mouse
- SwissProt: P07171 Rat
- Unigene: 65425 Human
- Unigene: 277665 Mouse
- Unigene: 3908 Rat
see all
Alternative names
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
- CALB antibody
- CALB1 antibody
- CALB1_HUMAN antibody
- Calbindin 1 28kDa antibody
- Calbindin antibody
- Calbindin D28 antibody
- D 28K antibody
- D-28K antibody
- D28K antibody
- OTTHUMP00000166027 antibody
- OTTHUMP00000225441 antibody
- RTVL H protein antibody
- Vitamin D dependent calcium binding protein antibody
- Vitamin D dependent calcium binding protein avian type antibody
- Vitamin D-dependent calcium-binding protein antibody
see all

Western blot - Anti-Calbindin antibody (ab49899)

All lanes : ab49899 at 1 µg/ml

Lane 1 : Brain (human) tissue lysate
Lane 2 : Brain (Mouse) Tissue lysate
Lane 3 : Brain (Rat) Tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 37 kDa, 51 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 30 seconds
Western blot - Anti-Calbindin antibody (ab49899)

All lanes : Anti-Calbindin antibody (ab49899) at 1 µg/ml

Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-Calbindin antibody (ab49899)

Calbindin - Neuronal Marker was immunoprecipitated using 0.5mg Mouse kidney whole tissue extract, 5µg of Rabbit polyclonal to Calbindin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse kidney whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49899.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 30kDa: Calbindin - Neuronal Marker.

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet

Publishing research using ab49899? Please let us know so that we can cite the reference in this datasheet.

ab49899 has been referenced in 2 publications.

Schludi MH et al. Spinal poly-GA inclusions in a C9orf72 mouse model trigger motor deficits and inflammation without neuron loss. Acta Neuropathol 134:241-254 (2017). PubMed: 28409281
Schmouth JF et al. Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice. BMC Biol 11:106 (2013). IHC . PubMed: 24124870

Western blot - Anti-Calbindin antibody (ab49899)

All lanes : ab49899 at 1 µg/ml

Lane 1 : Brain (human) tissue lysate
Lane 2 : Brain (Mouse) Tissue lysate
Lane 3 : Brain (Rat) Tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 37 kDa, 51 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 30 seconds
Western blot - Anti-Calbindin antibody (ab49899)

All lanes : Anti-Calbindin antibody (ab49899) at 1 µg/ml

Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-Calbindin antibody (ab49899)

Calbindin - Neuronal Marker was immunoprecipitated using 0.5mg Mouse kidney whole tissue extract, 5µg of Rabbit polyclonal to Calbindin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse kidney whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49899.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 30kDa: Calbindin - Neuronal Marker.