Anti-Calbindin antibody (ab156812)
Key features and details
- Goat polyclonal to Calbindin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Calbindin antibody
See all Calbindin primary antibodies -
Description
Goat polyclonal to Calbindin -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide:
KTFVDQYGQRDDGK
by a Cysteine residue linker, corresponding to internal sequence amino acids 59-72 of Human Calbindin (NP_004920.1). -
Positive control
- Human Brain (Cerebellum) lysates, Mouse brain lysate, Rat brain lysate
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General notes
ab156812 overlaps EF hand 2, but not any calcium binding site.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab156812 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
ab156812 overlaps EF hand 2, but not any calcium binding site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calbindin antibody (ab156812)Anti-Calbindin antibody (ab156812) at 0.03 µg/ml + Human Brain Cerebellum lysate (in RIPA buffer). at 35 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
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Western blot - Anti-Calbindin antibody (ab156812)All lanes : Anti-Calbindin antibody (ab156812) at 0.1 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : rat brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
