Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22625-61] to CACNA1C - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free
    See all CACNA1C primary antibodies

  • Description

    Rabbit monoclonal [EPR22625-61] to CACNA1C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse atrium, ventricle and colon tissue. IHC-Fr: Mouse cardiac muscle and cerebrum tissue.

  • General notes

    Ab256543 is the carrier-free version of ab234438. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256543 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)
    Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling CACNA1C with ab234438 at 1/500 (0.87 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. Positive staining on mouse cerebrum is observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

  • Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)
    Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle tissue labeling CACNA1C with ab234438 at 1/500 (0.87 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. Positive staining on mouse cardiac atrium myocytes and blood vessels throughout cardiac muscle (PMID: 26109061).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CACNA1C with ab234438 at 1/4000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of blood vessels, muscularis mucosa and muscularis externa in mouse colon is observed. The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)

    Immunohistochemical analysis of paraffin-embedded mouse ventricle tissue labeling CACNA1C with ab234438 at 1/2000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the endothelial cells of blood vessels in mouse ventricle is observed (PMID: 26109061). The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)

    Immunohistochemical analysis of paraffin-embedded mouse atrium tissue labeling CACNA1C with ab234438 at 1/4000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma membranes of atrial cardiomyocytes and endothelial cells of blood vessels is observed (PMID: 26109061). The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

  • Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)
    Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (ab256543)

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