Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20905-21] to ALPPL2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free
    See all ALPPL2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20905-21] to ALPPL2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PANC-1, Capan-1 and nES whole cell lysates.

  • General notes

    ab269957 is the carrier-free version of ab235581. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)
    Western blot - Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)
    All lanes : Anti-ALPPL2 antibody [EPR20905-21] (ab235581) at 1/1000 dilution

    Lane 1 : nES (human native embryonic stem cell), whole cell lysate
    Lane 2 : pES (human primed embryonic stem cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were kindly provided by Dr Shaorong Gao.

    140KD band is dimer (PMID: 23467613). 

    Exposure time: 5.5 seconds.

    These data were developed using the same antibody clone in a different  buffer formulation containing PBS, glycerol, BSA and sodium azide (ab235581).

  • Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)
    Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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