Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP263Y] to IRS1 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-IRS1 antibody [EP263Y] - BSA and Azide free
    See all IRS1 primary antibodies

  • Description

    Rabbit monoclonal [EP263Y] to IRS1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247286 is the carrier-free version of ab40777 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247286 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Anti-IRS1 antibody [EP263Y] (ab40777) at 1/10000 dilution (purified) + HEK293 whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 170 kDa
    Observed band size: 170 kDa



    This data was developed using ab40777, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)

    This data was developed using ab40777, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling IRS1 with purified ab40777 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunocytochemistry - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Immunocytochemistry - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    This data was developed using ab40777, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling IRS1 with purified ab40777 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used. Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
  • Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Anti-IRS1 antibody [EP263Y] (ab40777) (purified) + HeLa whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 170 kDa
    Observed band size: 170 kDa



    This data was developed using ab40777, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Flow Cytometry - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    This data was developed using ab40777, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling IRS1 with unpurified ab40777 at 1/90 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Anti-IRS1 antibody [EP263Y] (ab40777) at 1/1000 dilution (unpurified) + HeLa cell lysate at 10 µg

    Predicted band size: 170 kDa
    Observed band size: 170 kDa



    This data was developed using ab40777, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    This data was developed using ab40777, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labelling IRS1 with unpurified ab40777. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
    Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)

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