Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14780] to C4BPA - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-C4BPA antibody [EPR14780] - BSA and Azide free
    See all C4BPA primary antibodies

  • Description

    Rabbit monoclonal [EPR14780] to C4BPA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251309 is the carrier-free version of ab200345. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251309 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR14780

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)
    Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)
    Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution + Human plasma at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 30 seconds


    This data was developed using ab200345, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)
    Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)
    All lanes : Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 30 seconds


    This data was developed using ab200345, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)
    This data was developed using ab200345, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
    Note: Plasma staining on human transitional cell carcinoma of bladder tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)
    This data was developed using ab200345, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
    Note: No staining on human skeletal muscle tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)
    Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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