Anti-C2b antibody (ab66999)
Key features and details
- Rabbit polyclonal to C2b
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-C2b antibody -
Description
Rabbit polyclonal to C2b -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
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Positive control
- This antibody gave a positive signal in Mouse liver tissue lysate.
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General notes
This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedPrimary antibody notes
This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)Clonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 84 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 84 kDa).Target
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Relevance
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 12263 Mouse
- SwissProt: P21180 Mouse
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Alternative names
- C2 antibody
- C2b antibody
- CO2 antibody
see all
Images
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Western blot - Anti-C2b antibody (ab66999)Anti-C2b antibody (ab66999) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Mouse Complement C2 precursor (C2b) contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 97 kDa band observed is also comparable to the molecular weight seen with other commercially available antibodies to C2b.
Datasheets and documents
References (0)
ab66999 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-C2b antibody (ab66999)Anti-C2b antibody (ab66999) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Mouse Complement C2 precursor (C2b) contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 97 kDa band observed is also comparable to the molecular weight seen with other commercially available antibodies to C2b.