Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Tuesday March 09, 2021

Anti-C21orf33 antibody [EPR13213] (ab181366)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13213] to C21orf33
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Human

Product name

Anti-C21orf33 antibody [EPR13213]
See all C21orf33 primary antibodies
Description

Rabbit monoclonal [EPR13213] to C21orf33
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human C21orf33. The exact sequence is proprietary.
Database link: P30042
Positive control
- A549, HeLa, HepG2 and K562 cell lysates, Human heart tissue, Human liver tissue and HepG2 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13213
Isotype

IgG
Research areas

Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (ab181366)

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or 1XPBS (lane 2) labeling C21orf33 using ab181366 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (ab181366)

Immunofluorescent staining of HepG2 cells labeling C21orf33 using ab181366 at a 1/100 dilution (red) with DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C21orf33 using ab181366 at a 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C21orf33 using ab181366 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-C21orf33 antibody [EPR13213] (ab181366)

All lanes : Anti-C21orf33 antibody [EPR13213] (ab181366) at 1/5000 dilution

Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 25 kDa
Anti-C21orf33 antibody [EPR13213] (ab181366)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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