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Anti-C21orf33 antibody [EPR13213] (ab181366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13213] to C21orf33
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-C21orf33 antibody [EPR13213]
    See all C21orf33 primary antibodies

  • Description

    Rabbit monoclonal [EPR13213] to C21orf33

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human C21orf33. The exact sequence is proprietary.
    Database link: P30042

  • Positive control

    • A549, HeLa, HepG2 and K562 cell lysates, Human heart tissue, Human liver tissue and HepG2 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (ab181366)
    Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (ab181366)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or 1XPBS (lane 2) labeling C21orf33 using ab181366 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (ab181366)
    Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (ab181366)

    Immunofluorescent staining of HepG2 cells labeling C21orf33 using ab181366 at a 1/100 dilution (red) with DAPI nuclear staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C21orf33 using ab181366 at a 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C21orf33 using ab181366 at a 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-C21orf33 antibody [EPR13213] (ab181366)
    Western blot - Anti-C21orf33 antibody [EPR13213] (ab181366)
    All lanes : Anti-C21orf33 antibody [EPR13213] (ab181366) at 1/5000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 25 kDa

  • Anti-C21orf33 antibody [EPR13213] (ab181366)
    Anti-C21orf33 antibody [EPR13213] (ab181366)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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