Anti-c-Myc antibody (ab152146)
Key features and details
- Rabbit polyclonal to c-Myc
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Myc antibody
See all c-Myc primary antibodies -
Description
Rabbit polyclonal to c-Myc -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Myc Tag
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Positive control
- 293T cells transfected with Myc tag recombinant protein lysate; HeLa Myc tag transfected lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-c-Myc antibody (ab152146) at 1/5000 dilution
Lane 1 : Non-transfected 293T cell lysate
Lane 2 : Transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
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Immunofluorescence analysis of paraformaldehyde fixed HeLa Myc tag transfected cells, labeling Myc tag using ab152146 at 1/2500 dilution (green) and alpha tubulin filaments (red).
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Myc antibody Immunoprecipitation of Myc-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using:
1: Input, 15ug of HeLa transfected lysates.
2: normal rabbit IgG or
3: 2.5 ug of ab152146.
The precipitated protein was detected by ab152146 diluted at 1/10000.