Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free (ab254200)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22566-205] to c-Kit - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free
    See all c-Kit primary antibodies

  • Description

    Rabbit monoclonal [EPR22566-205] to c-Kit - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebellum and testis tissue; rat testis tissue.

  • General notes

    Ab254200 is the carrier-free version of ab231780. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254200 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free (ab254200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free (ab254200)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling c-Kit with ab231780 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on spermatogonia and Leydig cells of rat testis (PMID: 29324782) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    The section was incubated with ab231780 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231780).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free (ab254200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free (ab254200)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling c-Kit with ab231780 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on spermatogonia and Leydig cells of mouse testis (PMID: 29324782) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    The section was incubated with ab231780 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231780).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free (ab254200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free (ab254200)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling c-Kit with ab231780 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Positive staining in Purkinje cells of mouse cerebellum (PMID: 15100248) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    The section was incubated with ab231780 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231780).

  • Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free (ab254200)
    Anti-c-Kit antibody [EPR22566-205] - BSA and Azide free (ab254200)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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