Anti-c-Jun (phospho S243) antibody (ab28846)
Key features and details
- Rabbit polyclonal to c-Jun (phospho S243)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Jun (phospho S243) antibody
See all c-Jun primary antibodies -
Description
Rabbit polyclonal to c-Jun (phospho S243) -
Host species
Rabbit -
Specificity
c-Jun (Phospho-Ser243) Antibody detects endogenous levels of c-Jun only when phosphorylated at Ser243. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human c-Jun (phospho S243). Immunogen is in the range of aa 210 - 259
Database link: P05412
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against nonphosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Jun (phospho S243) antibody (ab28846)All lanes : Anti-c-Jun (phospho S243) antibody (ab28846) at 1/500 dilution
Lane 1 : HeLa cell lysate (5-30ug) - untreated
Lane 2 : HeLa cell lysate (5-30ug) - treated with UV light
Predicted band size: 36 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S243) antibody (ab28846)Ab28846, at a dilution of 1/50, staining c-Jun in paraffin embedded human breast carcinoma by Immunohistochemistry.
Left image: using c-Jun (phospho S243).
Right image: the same antibody preincubated with the corresponding synthesized phosphopeptide.
