Anti-c-Jun antibody (ab105186)
Key features and details
- Rabbit polyclonal to c-Jun
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-c-Jun antibody
See all c-Jun primary antibodies -
Description
Rabbit polyclonal to c-Jun -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 40 and 80 of Human c-Jun (NP_002219.1).
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Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab105186 was affinity purified using an epitope specific to c-Jun immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Jun antibody (ab105186)All lanes : Anti-c-Jun antibody (ab105186) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 36 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-c-Jun antibody (ab105186)Detection of c-Jun by Western Blot of Immunprecipitate.
ab105186 at 1µg/ml staining c-Jun in HeLa whole cell lysate immunoprecipitated using ab105186 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 90 seconds.
