Anti-BYSL antibody (ab251811)
Key features and details
- Rabbit polyclonal to BYSL
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BYSL antibody
See all BYSL primary antibodies -
Description
Rabbit polyclonal to BYSL -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment corresponding to Human BYSL aa 350-450.
Database link: Q13895 -
Positive control
- IHC-P: Human rectum, placenta, testis and lymphoid tissues. ICC/IF: A431 cells. WB: RT4, U-251 MG, NIH/3T3 and NBT-II cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BYSL antibody (ab251811)
Formalin-fixed, paraffin-embedded human lymphoid tissue stained for BYSL with ab251811 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BYSL antibody (ab251811)Formalin-fixed, paraffin-embedded human placenta tissue stained for BYSL with ab251811 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BYSL antibody (ab251811)Formalin-fixed, paraffin-embedded human testis tissue stained for BYSL with ab251811 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BYSL antibody (ab251811)Formalin-fixed, paraffin-embedded human rectum tissue stained for BYSL with ab251811 at a 1/200 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-BYSL antibody (ab251811)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for BYSL (green) using ab251811 at 4 µg/ml in ICC/IF. -
Western blot - Anti-BYSL antibody (ab251811)All lanes : Anti-BYSL antibody (ab251811) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II whole cell lysate
Predicted band size: 50 kDa
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Western blot - Anti-BYSL antibody (ab251811)All lanes : Anti-BYSL antibody (ab251811) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 50 kDaWestern blot analysis using ab251811 (A) shows a simillar staining pattern to an independent antibody (B).