Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)
![Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)](https://www.abcam.com/ps/products/255/ab255892/Images/ab255892-411636-anti-btk-antibody-10d11-western-blot-raji.jpg "Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [10D11] to BTK - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-BTK antibody [10D11] - BSA and Azide free
See all BTK primary antibodies -
Description
Mouse monoclonal [10D11] to BTK - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji, Ramos, K562 and NAMALWA whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255892 is the carrier-free version of ab254141. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255892 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
10D11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)All lanes : Anti-BTK antibody [10D11] (ab254141) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 3 : NAMALWA (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 6 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-5 : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 114 secondsThis image was produced using the same antibody clone but in a different buffer formulation, ab254141.
Blocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Negative control: Jurkat, MOLT-4 (PMID 24759210).
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![Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)](https://www.abcam.com/ps/products/255/ab255892/Images/ab255892-2-benefits-of-recombinant-antibodies.png)
Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)
