Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [10D11] to BTK - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BTK antibody [10D11] - BSA and Azide free
    See all BTK primary antibodies

  • Description

    Mouse monoclonal [10D11] to BTK - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji, Ramos, K562 and NAMALWA whole cell lysates.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255892 is the carrier-free version of ab254141. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255892 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)
    Western blot - Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)
    All lanes : Anti-BTK antibody [10D11] (ab254141) at 1/1000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 2 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 3 : NAMALWA (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 4 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 6 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-5 : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution


    Predicted band size: 76 kDa
    Observed band size: 76 kDa


    Exposure time: 114 seconds


    This image was produced using the same antibody clone but in a different buffer formulation, ab254141.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Negative control: Jurkat, MOLT-4 (PMID 24759210).

  • Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)
    Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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