Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRMS1 antibody [EPR7202] (ab134968)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7202] to BRMS1
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-BRMS1 antibody [EPR7202]
    See all BRMS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7202] to BRMS1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BRMS1 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, HepG2, and Human colon cancer lysates; Human colon and placenta tissues; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7202

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-BRMS1 antibody [EPR7202] (ab134968)
    Western blot - Anti-BRMS1 antibody [EPR7202] (ab134968)
    All lanes : Anti-BRMS1 antibody [EPR7202] (ab134968) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human colon cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BRMS1 antibody [EPR7202] (ab134968)
    Immunocytochemistry/ Immunofluorescence - Anti-BRMS1 antibody [EPR7202] (ab134968)

    Immunofluorescence staining of HepG2 cells with purified ab134968 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody [EPR7202] (ab134968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody [EPR7202] (ab134968)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling BRMS1 with ab134968 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody [EPR7202] (ab134968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody [EPR7202] (ab134968)

    Imunohistochemical analysis of paraffin-embedded Human placenta tissue labelling BRMS1 with ab134968 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody [EPR7202] (ab134968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody [EPR7202] (ab134968)

    Immunohistochemical analysis using ab134968 showing positive staining in Ovarian carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody [EPR7202] (ab134968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody [EPR7202] (ab134968)

    Immunohistochemical analysis using ab134968 showing positive staining in Breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-BRMS1 antibody [EPR7202] (ab134968)
    Anti-BRMS1 antibody [EPR7202] (ab134968)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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