Anti-BNP antibody [C10] (ab239510)
![Anti-BNP antibody [C10] (ab239510)](https://www.abcam.com/ps/products/239/ab239510/Images/ab239510-317845-anti-bnp-antibody-c10-western-blot.jpg "Anti-BNP antibody [C10] (ab239510)")
Key features and details
- Mouse monoclonal [C10] to BNP
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG2b
Overview
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Product name
Anti-BNP antibody [C10]
See all BNP primary antibodies -
Description
Mouse monoclonal [C10] to BNP -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Mouse BNP aa 1 to the C-terminus. Two N-terminal tags, His-tag and SUMO-tag. Expressed in E. coli. Full length mature chain without signal peptide.
Database link: P40753-1 -
Positive control
- WB: Recombinant mouse BNP protein; Rat heart and testis tissue. IHC-P: Mouse kidney, testis, liver and brain tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C10 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-BNP antibody [C10] (ab239510)All lanes : Anti-BNP antibody [C10] (ab239510) at 2 µg/ml
Lane 1 : Rat heart tissue lysate
Lane 2 : Rat testis tissue lysate
Secondary
All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [C10] (ab239510)](https://www.abcam.com/ps/products/239/ab239510/Images/ab239510-317843-anti-bnp-antibody-c10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [C10] (ab239510)Formalin-fixed, paraffin-embedded mouse cerebrum tissue stained for BNP with ab239510 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [C10] (ab239510)](https://www.abcam.com/ps/products/239/ab239510/Images/ab239510-317842-anti-bnp-antibody-c10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [C10] (ab239510)Formalin-fixed, paraffin-embedded mouse liver tissue stained for BNP with ab239510 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [C10] (ab239510)](https://www.abcam.com/ps/products/239/ab239510/Images/ab239510-317841-anti-bnp-antibody-c10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [C10] (ab239510)Formalin-fixed, paraffin-embedded mouse testis tissue stained for BNP with ab239510 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [C10] (ab239510)](https://www.abcam.com/ps/products/239/ab239510/Images/ab239510-317840-anti-bnp-antibody-c10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [C10] (ab239510)Formalin-fixed, paraffin-embedded mouse kidney tissue stained for BNP with ab239510 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
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![Western blot - Anti-BNP antibody [C10] (ab239510)](https://www.abcam.com/ps/products/239/ab239510/Images/ab239510-317844-anti-bnp-antibody-c10-western-blot.jpg)
Western blot - Anti-BNP antibody [C10] (ab239510)Anti-BNP antibody [C10] (ab239510) at 5 µg/ml + Recombinant mouse BNP protein
