Anti-Bmi1 antibody [LLBmi1-1] - BSA and Azide free (ab222600)
Key features and details
- Mouse monoclonal [LLBmi1-1] to Bmi1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bmi1 antibody [LLBmi1-1] - BSA and Azide free
See all Bmi1 primary antibodies -
Description
Mouse monoclonal [LLBmi1-1] to Bmi1 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Full length protein.
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: MOLT4, Hek293, MEF1
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General notes
ab222600 is a PBS only version of ab155069.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This monoclonal antibody to Bmi1 has been knockout validated in Western blot. The expected band for Bmi1 was observed in wild type cells and the band was not seen in knockout cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
LLBmi1-1 -
Isotype
IgG -
Research areas
Images
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Bmi1 knockout HAP1 cell lysate (20 µg)
Lane 3: U2OS cell lysate (20 µg)
Lane 4: Molt-4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab155069 observed at 42 kDa. Red - loading control, ab176560, observed at 52 kDa.ab155069 was shown to specifically recognize Bmi1. Wild-type and Bmi1 knockout samples were subjected to SDS-PAGE. ab155069 and ab176560 (loading control to alpha tubulin) were diluted at 2.5 ug/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different formulation containing PBS and Azide (ab155069).