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Cancer Cell cycle Cell differentiation

Anti-Bmi1 antibody [LLBmi1-1] - BSA and Azide free (ab222600)

Anti-Bmi1 antibody [LLBmi1-1] - BSA and Azide free (ab222600)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [LLBmi1-1] to Bmi1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Bmi1 antibody [LLBmi1-1] - BSA and Azide free
    See all Bmi1 primary antibodies
  • Description

    Mouse monoclonal [LLBmi1-1] to Bmi1 - BSA and Azide free
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length protein.

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: MOLT4, Hek293, MEF1
  • General notes

    ab222600 is a PBS only version of ab155069.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This monoclonal antibody to Bmi1 has been knockout validated in Western blot. The expected band for Bmi1 was observed in wild type cells and the band was not seen in knockout cells.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    LLBmi1-1
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC2
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Other

Images

  • Western blot - Anti-Bmi1 antibody [LLBmi1-1] - BSA and Azide free (ab222600)
    Western blot - Anti-Bmi1 antibody [LLBmi1-1] - BSA and Azide free (ab222600)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Bmi1 knockout HAP1 cell lysate (20 µg)
    Lane 3: U2OS cell lysate (20 µg)
    Lane 4: Molt-4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab155069 observed at 42 kDa. Red - loading control, ab176560, observed at 52 kDa.

    ab155069 was shown to specifically recognize Bmi1. Wild-type and Bmi1 knockout samples were subjected to SDS-PAGE. ab155069 and ab176560 (loading control to alpha tubulin) were diluted at 2.5 ug/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different formulation containing PBS and Azide (ab155069).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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