Anti-Bmi1 antibody (ab115251)
Key features and details
- Goat polyclonal to Bmi1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Bmi1 antibody
See all Bmi1 primary antibodies -
Description
Goat polyclonal to Bmi1 -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Orangutan -
Immunogen
Synthetic peptide:
DSGSDKANSPAGG
, corresponding to amino acids 252-264 of Human Bmi1 -
Positive control
- Recombinant Human Bmi1 protein (ab114169) can be used as a positive control in WB. Human Skeletal Muscle tissue
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with ab115251 at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
-
ab115251 staining Bmi1 in Mouse Pancreatic intraepithelial neoplasia tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked with 10% BSA for 60 minutes at 26°C and antigen retrieval was by heat mediation in Tris-based buffer. The sample was incubated with primary antibody (1/200 in 10% BSA in TBST) at 4°C for 16 hours. A HRP-conjugated Donkey anti-goatpolyclonal (1/1000) was used as the secondary antibody.