Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20235] to Biglycan - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Biglycan antibody [EPR20235] - BSA and Azide free
    See all Biglycan primary antibodies

  • Description

    Rabbit monoclonal [EPR20235] to Biglycan - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251501 is the carrier-free version of ab209234. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251501 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)
    This data was developed using ab209234, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Biglycan with ab209234 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Specific extracellular matrix staining on peritubular myoid cells and leydig cells of human testis is observed [PMID: 7626709]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)
    This data was developed using ab209234, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Biglycan with ab209234 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on basement membrane and blood vessel of human kidney is observed [PMID: 11012890]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)
    This data was developed using ab209234, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Biglycan with ab209234 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on stromal cells of human bladder cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)
    This data was developed using ab209234, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling Biglycan with ab209234 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on stromal cells of human thyroid cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)
    Anti-Biglycan antibody [EPR20235] - BSA and Azide free (ab251501)

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