Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bid antibody [Y8] (ab32060)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y8] to Bid
  • Suitable for: ICC, WB, IHC-P, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Bid antibody [Y8]
    See all Bid primary antibodies

  • Description

    Rabbit monoclonal [Y8] to Bid

  • Host species

    Rabbit

  • Specificity

    This antibody does not cross-react with other Bcl-2 family members.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Bid aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Jurkat cell lysate and human prostate carcinoma.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Bid antibody [Y8] (ab32060)
    Western blot - Anti-Bid antibody [Y8] (ab32060)
    Anti-Bid antibody [Y8] (ab32060) at 1/1000 dilution + Jurkat cell lysate

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody [Y8] (ab32060)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody [Y8] (ab32060)

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32060 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-Bid antibody [Y8] (ab32060)
    Immunocytochemistry - Anti-Bid antibody [Y8] (ab32060)
    Immunocytochemistry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling Bid with purified ab32060 at 1/500 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunoprecipitation - Anti-Bid antibody [Y8] (ab32060)
    Immunoprecipitation - Anti-Bid antibody [Y8] (ab32060)

    Purified ab32060 at 1/50 dilution (2µg) immunoprecipitating Bid in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab32060 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32060 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 22 kDa

  • Flow Cytometry - Anti-Bid antibody [Y8] (ab32060)
    Flow Cytometry - Anti-Bid antibody [Y8] (ab32060)
    Overlay histogram showing HeLa cells stained with ab32060 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32060, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-Bid antibody [Y8] (ab32060)
    Western blot - Anti-Bid antibody [Y8] (ab32060) Image courtesy of an anonymous Abreview.
    All lanes : Anti-Bid antibody [Y8] (ab32060) at 1/500 dilution

    Lanes 1-2 : Whole cell lysate prepared from breast cancer clinical extract
    Lane 3 : Whole cell lysate prepared from MCF7 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG-HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 25 minutes

    See Abreview

  • Anti-Bid antibody [Y8] (ab32060)
    Anti-Bid antibody [Y8] (ab32060)

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