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Anti-Bid antibody - Carboxyterminal end (ab62469)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Bid antibody - Carboxyterminal end (ab62469)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Bid - Carboxyterminal end
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-Bid antibody - Carboxyterminal end
    See all Bid primary antibodies
  • Description

    Rabbit polyclonal to Bid - Carboxyterminal end
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with Bid
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    A 14 amino acid synthetic peptide from near the carboxy-terminus of human Bid

  • Positive control

    • Mouse lung cell lysates and mouse lung tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-Bid antibody - Carboxyterminal end (ab62469)
    Western blot - Anti-Bid antibody - Carboxyterminal end (ab62469)
    Lane 1 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 0.5 µg/ml
    Lane 2 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 1 µg/ml
    Lane 3 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 2 µg/ml

    All lanes : Mouse lung cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Anti rabbit IgG secondary antibody

    Predicted band size: 22 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody - Carboxyterminal end (ab62469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody - Carboxyterminal end (ab62469)
    Immunohistochemical staining of mouse lung tissue using ab62469 antibody at 2 µg/ml.
  • Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody - Carboxyterminal end (ab62469)
    Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody - Carboxyterminal end (ab62469)
    Immunofluorescence of Bid in Mouse Lung cells using ab62469 at 10 ug/ml.

  • Western blot - Anti-Bid antibody - Carboxyterminal end (ab62469)
    Western blot - Anti-Bid antibody - Carboxyterminal end (ab62469) Courtesy of an Abreview submitted by Christian Marx
    All lanes : Anti-Bid antibody - Carboxyterminal end (ab62469) at 1/1000 dilution

    Lane 1 : HCT116 colon cancer cell line treated with DMSO (control)
    Lane 2 : HCT116 colon cancer cell line treated with 2 µM SAHA for 24 hrs

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa


    Exposure time: 10 seconds

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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