Anti-Bid antibody - Carboxyterminal end (ab62469)
Key features and details
- Rabbit polyclonal to Bid - Carboxyterminal end
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Bid antibody - Carboxyterminal end
See all Bid primary antibodies -
Description
Rabbit polyclonal to Bid - Carboxyterminal end -
Host species
Rabbit -
Specificity
This antibody reacts with Bid -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
A 14 amino acid synthetic peptide from near the carboxy-terminus of human Bid
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Positive control
- Mouse lung cell lysates and mouse lung tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Lane 1 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 0.5 µg/ml
Lane 2 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 1 µg/ml
Lane 3 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 2 µg/ml
All lanes : Mouse lung cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit IgG secondary antibody
Predicted band size: 22 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody - Carboxyterminal end (ab62469)Immunohistochemical staining of mouse lung tissue using ab62469 antibody at 2 µg/ml.
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Immunofluorescence of Bid in Mouse Lung cells using ab62469 at 10 ug/ml.
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Western blot - Anti-Bid antibody - Carboxyterminal end (ab62469) Courtesy of an Abreview submitted by Christian MarxAll lanes : Anti-Bid antibody - Carboxyterminal end (ab62469) at 1/1000 dilution
Lane 1 : HCT116 colon cancer cell line treated with DMSO (control)
Lane 2 : HCT116 colon cancer cell line treated with 2 µM SAHA for 24 hrs
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Exposure time: 10 seconds