Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AA10] to beta III Tubulin - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-beta III Tubulin antibody [AA10] - BSA and Azide free
    See all beta III Tubulin primary antibodies

  • Description

    Mouse monoclonal [AA10] to beta III Tubulin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat brain tissue lysate. SH-SY5Y and Neuro-2a whole cell lysate. IHC-P: Human tonsil and colon tissue. Human, mouse and rat cerebellum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255737 is the carrier-free version of ab231083. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    AA10

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    Western blot - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    All lanes : Anti-beta III Tubulin antibody [AA10] (ab231083) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 50 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Human liver (PMID:20191564).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)

    Immunohistochemical analysis of paraffin-embedded human tonstil tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human tonsil. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

  • Western blot - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    Western blot - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    All lanes : Anti-beta III Tubulin antibody [AA10] (ab231083) at 1/1000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 2-4 : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 seconds; Lane 2-4: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat cerebellum. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebellum. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cerebellum. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human colon. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

  • Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)
    Anti-beta III Tubulin antibody [AA10] - BSA and Azide free (ab255737)

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