Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
![Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)](https://www.abcam.com/ps/products/29/ab29037/Images/ab29037-40186-anti-beta-dystroglycan-phospho-y892-antibody-m117-western-blot.jpg "Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)")
Key features and details
- Mouse monoclonal [M117] to beta Dystroglycan (phospho Y892)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta Dystroglycan (phospho Y892) antibody [M117]
See all beta Dystroglycan primary antibodies -
Description
Mouse monoclonal [M117] to beta Dystroglycan (phospho Y892) -
Host species
Mouse -
Specificity
Ab29037 recognises beta Dystroglycan (phospho Y892). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 892 of human dystroglycan.
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Positive control
- HepG2 cells or HeLa cells treated with pervanadate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M117 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)All lanes : Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
Lane 1 : Lysates from HepG2 cells (untreated)
Lane 2 : Lysates from HepG2 cells treated with pervanadate (1 mM) for 30 min
Lysates/proteins at 20 µg per lane.
Predicted band size: 97 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Dystroglycan is expressed as a precursor protein (97kDa) that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa. -
![Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)](https://www.abcam.com/ps/products/29/ab29037/Images/ab29037-40185-anti-beta-dystroglycan-phospho-y892-antibody-m117-western-blot.jpg)
Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-beta-dystroglycan (lanes 1 & 2) and anti-beta-dystroglycan (Tyr-892) (lanes 3 & 4).
