Anti-beta Catenin (phospho S552) antibody [1HCLC] (ab277785)
![Anti-beta Catenin (phospho S552) antibody [1HCLC] (ab277785)](https://www.abcam.com/ps/products/277/ab277785/Images/ab277785-2-antiPhosphobetaCateninS552recombinantpolyclonalantibody1hclcWB.jpg "Anti-beta Catenin (phospho S552) antibody [1HCLC] (ab277785)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [1HCLC] to beta Catenin (phospho S552)
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-beta Catenin (phospho S552) antibody [1HCLC]
See all beta Catenin primary antibodies -
Description
Rabbit recombinant multiclonal [1HCLC] to beta Catenin (phospho S552) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide aa 549-556 (phospho S552).
Database link: P35222 -
General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
1HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-beta Catenin antibody (ab277785)All lanes : Anti-beta Catenin (phospho S552) antibody [1HCLC] (ab277785) at 2 µg/ml
Lane 1 : SW480 (human colorectal adenocarcinoma cell line) whole cell extract
Lane 2 : SW480 (human colorectal adenocarcinoma cell line) whole cell extract (treated with MG132, 25 uM for 4 hrs)
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract (treated with MG132, 25 uM for 4 hrs)
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell extract (treated with MG132, 25 uM for 4 hrs)
Predicted band size: 85 kDa
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Immunocytochemistry - Anti-beta Catenin antibody (ab277785)For immunofluorescence analysis, SW480 cells were fixed and permeabilized for detection of endogenous Beta-Catenin pS552 using Anti-Beta-Catenin pS552 Recombinant Rabbit Multiclonal Antibody (ab277785, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI, and Alexa Fluor® 555 Rhodamine Phalloidin (1/300) was used for cytoskeletal F-actin (red) staining. Detection and localization of Beta-Catenin pS552 (green) in the cytoplasm can be clearly observed in cells treated with the proteasome inhibitor Mμ-132 (25 uM, 4 h) as compared to untreated cells. Antibody specificity was demonstrated by competition with the Beta-Catenin pS552 peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.
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![Anti-beta Catenin antibody [1HCLC] (ab277785)](https://www.abcam.com/ps/products/277/ab277785/Images/ab277785-3-benefits-of-recombinant-antibodies.jpg)
Anti-beta Catenin antibody [1HCLC] (ab277785)