Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (ab273577)
![Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (ab273577)](https://www.abcam.com/ps/products/273/ab273577/Images/ab273577-394422-antibend3antibodyepr2103860westernblothepg2.jpg "Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (ab273577)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21038-60] to BEND3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free
See all BEND3 primary antibodies -
Description
Rabbit monoclonal [EPR21038-60] to BEND3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with BEND3(WT) expression vector containing a GFP-His-tag®, whole cell lysate; HepG2, HeLa, HEK-293T, F9, U-2 OS and NCCIT whole cell lysates.
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General notes
ab273577 is the carrier-free version of ab215708. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21038-60 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (ab273577)All lanes : Anti-BEND3 antibody [EPR21038-60] (ab215708) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 4 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
Lane 6 : NCCIT (human pluripotent embryonic carcinoma epithelial cell), whole cell lysate
Lane 7 : HeLa treated with 10 uM MG-132 for 4 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
50 kDa unknown bands are observed.
Exposure times: Lanes 1-3: 158 seconds; Lanes 4-8: 180 seconds .
Thise data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab215708).
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![Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (ab273577)](https://www.abcam.com/ps/products/273/ab273577/Images/ab273577-2-benefits-of-recombinant-antibodies.png)
Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (ab273577)