Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] - BSA and Azide free (ab247378)
![Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] - BSA and Azide free (ab247378)](https://www.abcam.com/ps/products/247/ab247378/Images/ab247378-397914-anti-bcr-phospho-y177-antibody-epr576-2-y-bsa-and-azide-free-western-blot.jpg "Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] - BSA and Azide free (ab247378)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR576(2)Y] to Bcr (phospho Y177) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] - BSA and Azide free
See all Bcr primary antibodies -
Description
Rabbit monoclonal [EPR576(2)Y] to Bcr (phospho Y177) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247378 is the carrier-free version of ab68216 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247378 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR576(2)Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] - BSA and Azide free (ab247378)All lanes : Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216) at 1/1000 dilution
Lane 1 : 3T3 cell lysate, membrane un-treated
Lane 2 : 3T3 cell lysate, membrane treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 143 kDa
Observed band size: 153 kDa why is the actual band size different from the predicted?This data was developed using ab68216, the same antibody clone in a different buffer formulation.
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![Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] - BSA and Azide free (ab247378)](https://www.abcam.com/ps/products/247/ab247378/Images/ab247378-2-benefits-of-recombinant-antibodies.png)
Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] - BSA and Azide free (ab247378)
