Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10355] to cGMP - BSA and Azide free
  • Suitable for: ELISA
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-cGMP antibody [EPR10355] - BSA and Azide free
    See all cGMP primary antibodies

  • Description

    Rabbit monoclonal [EPR10355] to cGMP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISAmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Chemical/ Small Molecule corresponding to cGMP conjugated to keyhole limpet haemocyanin.

  • General notes

    ab249503 is the carrier-free version of ab169753 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249503 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The product is specific for cGMP and as tested it does not cross-react with cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP and cCMP.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)
    ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)
    This data was developed using ab169753, the same antibody clone in a different buffer formulation.2 &microg/ml of Goat anti-rabbit IgG (50 &microl/well) was coated into 96-wells. After washing with 0.1 % Tween-20, 0.1 &microg/ml of ab169753 (50 &microl/well) was added and incubated for 1 hour. After washing, 0, 8, 48, 64, 96, 128, 256 and 1000 ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP and cCMP (25 &microl/well) were added along with 25 &microl of the 1/50 diluted HRP conjugated cGMP. After washing, TMB was used to develop the color.
  • ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)
    ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)
    This data was developed using ab169753, the same antibody clone in a different buffer formulation.2&microg/ml of goat-anti-rabbit IgG (50&microl/well) was coated into 96-wells. After washing with 0.1% Tween-20, 0.2&microg/ml of ab169753 (50 &microl / well) was added and incubated for 1hr. Then 62.5ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP and H2O (25 &microl / well each) were added, respectively. Finally 25 &microl of 1/50 diluted HRP conjugated cGMP was added in each well. TMB was used to develop the color.
  • Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)
    Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)

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