Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)
![Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)](https://www.abcam.com/ps/products/249/ab249503/Images/ab249503-2-anti-cgmp-antibody-epr10355-bsa-and-azide-free-elisa.jpg "Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10355] to cGMP - BSA and Azide free
- Suitable for: ELISA
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-cGMP antibody [EPR10355] - BSA and Azide free
See all cGMP primary antibodies -
Description
Rabbit monoclonal [EPR10355] to cGMP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ELISAmore details
Unsuitable for: IP or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Chemical/ Small Molecule corresponding to cGMP conjugated to keyhole limpet haemocyanin.
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General notes
ab249503 is the carrier-free version of ab169753 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249503 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
The product is specific for cGMP and as tested it does not cross-react with cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP and cCMP.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10355 -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)This data was developed using ab169753, the same antibody clone in a different buffer formulation.2 µg/ml of Goat anti-rabbit IgG (50 µl/well) was coated into 96-wells. After washing with 0.1 % Tween-20, 0.1 µg/ml of ab169753 (50 µl/well) was added and incubated for 1 hour. After washing, 0, 8, 48, 64, 96, 128, 256 and 1000 ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP and cCMP (25 µl/well) were added along with 25 µl of the 1/50 diluted HRP conjugated cGMP. After washing, TMB was used to develop the color.
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![ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)](https://www.abcam.com/ps/products/249/ab249503/Images/ab249503-1-anti-cgmp-antibody-epr10355-bsa-and-azide-free-elisa.png)
ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)This data was developed using ab169753, the same antibody clone in a different buffer formulation.2µg/ml of goat-anti-rabbit IgG (50µl/well) was coated into 96-wells. After washing with 0.1% Tween-20, 0.2µg/ml of ab169753 (50 µl / well) was added and incubated for 1hr. Then 62.5ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP and H2O (25 µl / well each) were added, respectively. Finally 25 µl of 1/50 diluted HRP conjugated cGMP was added in each well. TMB was used to develop the color. -
![Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)](https://www.abcam.com/ps/products/249/ab249503/Images/ab249503-3-benefits-of-recombinant-antibodies.png)
Anti-cGMP antibody [EPR10355] - BSA and Azide free (ab249503)
