Anti-BCL9 antibody (ab37305)
Key features and details
- Rabbit polyclonal to BCL9
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BCL9 antibody -
Description
Rabbit polyclonal to BCL9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human BCL9 aa 800-900 conjugated to keyhole limpet haemocyanin.
(Peptide available asab37304)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab37305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 2 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 140 kDa).Can be blocked with Human BCL9 peptide (ab37304). ICC/IF Use a concentration of 1 µg/ml. Target
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Relevance
BCL9 is involved in signal transduction through the Wnt pathway. It promotes beta-catenin's transcriptional activity. BCL9 is associated with B-cell acute lymphoblastic leukemia. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies (referenced from swissprot and entrez gene). -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 607 Human
- Entrez Gene: 77578 Mouse
- Omim: 602597 Human
- SwissProt: O00512 Human
- SwissProt: Q1JQ81 Human
- SwissProt: Q5T489 Human
- SwissProt: Q9D219 Mouse
- Unigene: 415209 Human
see all -
Alternative names
- B-cell CLL/lymphoma 9 antibody
- BCL 9 antibody
- Legless homolog antibody
- LGS antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BCL9 antibody (ab37305)
ab37305 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37305 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
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Western blot - Anti-BCL9 antibody (ab37305)All lanes : Anti-BCL9 antibody (ab37305) at 2 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Protocols
References (15)
ab37305 has been referenced in 15 publications.
- Salazar VS et al. Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche. Elife 8:N/A (2019). PubMed: 30735122
- Wang L et al. Mechanism of Low Expression of miR-30a-5p on Epithelial-Mesenchymal Transition and Metastasis in Ovarian Cancer. DNA Cell Biol 38:341-351 (2019). PubMed: 30839226
- Soe MT et al. Immunohistochemical Mapping of Bcl9 Using Two Antibodies that Recognize Different Epitopes Is Useful to Characterize Juvenile Development of Hepatocellular Carcinoma in Myanmar. Acta Histochem Cytochem 52:9-17 (2019). PubMed: 30923411
- Feng M et al. Pharmacological inhibition of ß-catenin/BCL9 interaction overcomes resistance to immune checkpoint blockades by modulating Treg cells. Sci Adv 5:eaau5240 (2019). PubMed: 31086813
- Miao Y et al. miR-30a inhibits breast cancer progression through the Wnt/ß-catenin pathway. Int J Clin Exp Pathol 12:241-250 (2019). PubMed: 31933739
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BCL9 antibody (ab37305)
ab37305 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37305 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
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Western blot - Anti-BCL9 antibody (ab37305)All lanes : Anti-BCL9 antibody (ab37305) at 2 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
