Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Bcl2-L-13 antibody [EP10625] (ab203516)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP10625] to Bcl2-L-13
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Bcl2-L-13 antibody [EP10625]
See all Bcl2-L-13 primary antibodies
Description

Rabbit monoclonal [EP10625] to Bcl2-L-13
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Raji, K562, THP-1 and HeLa cell lysates. IHC-P: Human pancreas and tonsil tissues; Mouse cardiac muscle tissue; Rat kidney tissue.
General notes
Previously labelled as Bcl rambo.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP10625
Isotype

IgG
Research areas

Western blot - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)

Anti-Bcl2-L-13 antibody [EP10625] (ab203516) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 85 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes
Western blot - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)

All lanes : Anti-Bcl2-L-13 antibody [EP10625] (ab203516) at 1/5000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : THP-1 (Human monocytic leukemia cell line) cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and nuclear staining on human pancreas tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and nuclear staining on human tonsil tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and nuclear staining on mouse cardiac muscle tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Bcl2-L-13 antibody [EP10625] (ab203516)

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