Anti-Bcl2-L-13 antibody [EP10625] (ab203516)
![Anti-Bcl2-L-13 antibody [EP10625] (ab203516)](https://www.abcam.com/ps/products/203/ab203516/Images/ab203516-259351-anti-bcl2-l-13-antibody-ep10625-western-blot.jpg "Anti-Bcl2-L-13 antibody [EP10625] (ab203516)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP10625] to Bcl2-L-13
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Bcl2-L-13 antibody [EP10625]
See all Bcl2-L-13 primary antibodies -
Description
Rabbit monoclonal [EP10625] to Bcl2-L-13 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatHumanWB MouseHuman
-
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Raji, K562, THP-1 and HeLa cell lysates. IHC-P: Human pancreas and tonsil tissues; Mouse cardiac muscle tissue; Rat kidney tissue.
-
General notes
Previously labelled as Bcl rambo.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP10625 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)Anti-Bcl2-L-13 antibody [EP10625] (ab203516) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
-
![Western blot - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)](https://www.abcam.com/ps/products/203/ab203516/Images/ab203516-259350-anti-bcl2-l-13-antibody-ep10625-western-blot.jpg)
Western blot - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)All lanes : Anti-Bcl2-L-13 antibody [EP10625] (ab203516) at 1/5000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : THP-1 (Human monocytic leukemia cell line) cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)](https://www.abcam.com/ps/products/203/ab203516/Images/ab203516-259348-anti-bcl2-l-13-antibody-ep10625-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic and nuclear staining on human pancreas tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)](https://www.abcam.com/ps/products/203/ab203516/Images/ab203516-259347-anti-bcl2-l-13-antibody-ep10625-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic and nuclear staining on human tonsil tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)](https://www.abcam.com/ps/products/203/ab203516/Images/ab203516-259346-anti-bcl2-l-13-antibody-ep10625-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic and nuclear staining on mouse cardiac muscle tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)](https://www.abcam.com/ps/products/203/ab203516/Images/ab203516-259345-anti-bcl2-l-13-antibody-ep10625-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] (ab203516)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic and nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Bcl2-L-13 antibody [EP10625] (ab203516)](https://www.abcam.com/ps/products/203/ab203516/Images/ab203516-8-benefits-of-recombinant-antibodies.png)
Anti-Bcl2-L-13 antibody [EP10625] (ab203516)
