Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3567] to BAG2
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-BAG2 antibody [EPR3567]
See all BAG2 primary antibodies
Description

Rabbit monoclonal [EPR3567] to BAG2
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human BAG2 aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- WB: Jurkat, HeLa and HepG2 cell lysates. IHC-P: Human ovarian carcinoma tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3567
Isotype

IgG
Research areas

Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)

All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BAG2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 24 kDa
Observed band size: 25 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab79406 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa.

ab79406 Anti-BAG2 antibody [EPR3567] was shown to specifically react with BAG2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265907 (knockout cell lysate ab257369) was used. Wild-type and BAG2 knockout samples were subjected to SDS-PAGE. ab79406 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAG2 antibody [EPR3567] (ab79406)

ab79406, at 1/100 dilution, staining BAG2 in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-BAG2 antibody [EPR3567] (ab79406)

All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/5000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa
Anti-BAG2 antibody [EPR3567] (ab79406)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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