Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BAF60C antibody [EPR5538(2)] (ab171075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5538(2)] to BAF60C
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BAF60C antibody [EPR5538(2)]
    See all BAF60C primary antibodies

  • Description

    Rabbit monoclonal [EPR5538(2)] to BAF60C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human BAF60C aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q6STE5

  • Positive control

  • General notes

    Previously labelled as SMARCD3.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BAF60C antibody [EPR5538(2)] (ab171075)
    Western blot - Anti-BAF60C antibody [EPR5538(2)] (ab171075)
    All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] (ab171075)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] (ab171075)

    Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue labeling BAF60C with ab171075 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] (ab171075)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] (ab171075)

    Immunohistochemical analysis of paraffin-embedded human trachea tissue labeling BAF60C with ab171075 at 1/10 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-BAF60C antibody [EPR5538(2)] (ab171075)
    Immunoprecipitation - Anti-BAF60C antibody [EPR5538(2)] (ab171075)

    Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab171075 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.



    All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/10 dilution

    Lane 1 : Immunoprecipitation pellet from HeLa cell lysate
    Lane 2 : 1x PBS

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG
  • Anti-BAF60C antibody [EPR5538(2)] (ab171075)
    Anti-BAF60C antibody [EPR5538(2)] (ab171075)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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