Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-BAF60C antibody [EPR5538(2)] (ab171075)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5538(2)] to BAF60C
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-BAF60C antibody [EPR5538(2)]
See all BAF60C primary antibodies
Description

Rabbit monoclonal [EPR5538(2)] to BAF60C
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

within Human BAF60C aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q6STE5
Positive control
- Purchase matching WB positive control:Recombinant Human BAF60C protein
- HeLa, JAR, C6 and NIH/3T3 cell lysates, paraffin-embedded endometrial adenocarcinoma tissue, paraffin-embedded trachea tissue.
General notes
Previously labelled as SMARCD3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5538(2)
Isotype

IgG
Research areas

Western blot - Anti-BAF60C antibody [EPR5538(2)] (ab171075)

All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : C6 cell lysate
Lane 4 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] (ab171075)

Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue labeling BAF60C with ab171075 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] (ab171075)

Immunohistochemical analysis of paraffin-embedded human trachea tissue labeling BAF60C with ab171075 at 1/10 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-BAF60C antibody [EPR5538(2)] (ab171075)

Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab171075 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/10 dilution

Lane 1 : Immunoprecipitation pellet from HeLa cell lysate
Lane 2 : 1x PBS

Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Anti-BAF60C antibody [EPR5538(2)] (ab171075)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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