Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

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Anti-Azurocidin antibody [EPR9503] (ab181989)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9503] to Azurocidin
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-Azurocidin antibody [EPR9503]
See all Azurocidin primary antibodies
Description

Rabbit monoclonal [EPR9503] to Azurocidin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HUVEC, 293 cell lysate; Human spleen lysate; human spleen tissue and Human lung tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9503
Isotype

IgG
Research areas

Western blot - Anti-Azurocidin antibody [EPR9503] (ab181989)

All lanes : Anti-Azurocidin antibody [EPR9503] (ab181989) at 1/10000 dilution

Lane 1 : HUVEC cell lysate
Lane 2 : 293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Blocking/dilution buffer and concentration: 5% NFDM/TBST
Western blot - Anti-Azurocidin antibody [EPR9503] (ab181989)

Anti-Azurocidin antibody [EPR9503] (ab181989) at 1/2000 dilution + Human spleen lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 26 kDa

Blocking/dilution buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] (ab181989)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] (ab181989)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Azurocidin antibody [EPR9503] (ab181989)

Flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Azurocidin using ab181989 at a 1/40 dilution, or rabbit monoclonal IgG as isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.
Anti-Azurocidin antibody [EPR9503] (ab181989)

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