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Anti-Azurocidin antibody [EPR9503] (ab181989)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9503] to Azurocidin
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Azurocidin antibody [EPR9503]
    See all Azurocidin primary antibodies

  • Description

    Rabbit monoclonal [EPR9503] to Azurocidin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HUVEC, 293 cell lysate; Human spleen lysate; human spleen tissue and Human lung tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Azurocidin antibody [EPR9503] (ab181989)
    Western blot - Anti-Azurocidin antibody [EPR9503] (ab181989)
    All lanes : Anti-Azurocidin antibody [EPR9503] (ab181989) at 1/10000 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa



    Blocking/dilution buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Azurocidin antibody [EPR9503] (ab181989)
    Western blot - Anti-Azurocidin antibody [EPR9503] (ab181989)
    Anti-Azurocidin antibody [EPR9503] (ab181989) at 1/2000 dilution + Human spleen lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 26 kDa



    Blocking/dilution buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] (ab181989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] (ab181989)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted  (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] (ab181989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] (ab181989)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Azurocidin with ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Azurocidin antibody [EPR9503] (ab181989)
    Flow Cytometry - Anti-Azurocidin antibody [EPR9503] (ab181989)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Azurocidin using ab181989 at a 1/40 dilution, or rabbit monoclonal IgG as isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

  • Anti-Azurocidin antibody [EPR9503] (ab181989)
    Anti-Azurocidin antibody [EPR9503] (ab181989)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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