Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)
![Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)](https://www.abcam.com/ps/products/251/ab251145/Images/ab251145-7-anti-atp6v1b1-antibody-epr16400-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif "Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16400] to ATP6V1B1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free
See all ATP6V1B1 primary antibodies -
Description
Rabbit monoclonal [EPR16400] to ATP6V1B1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab251145 is the carrier-free version of ab192612. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251145 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16400 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)
This data was developed using ab192612, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP6V1B1 with ab192612 at 1/500 dilution (1.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)](https://www.abcam.com/ps/products/251/ab251145/Images/ab251145-5-anti-atp6v1b1-antibody-epr16400-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)This data was developed using ab192612, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ATP6V1B1 with ab192612 at 1/500 dilution (1.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)](https://www.abcam.com/ps/products/251/ab251145/Images/ab251145-4-anti-atp6v1b1-antibody-epr16400-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)This data was developed using ab192612, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP6V1B1 with ab192612 at 1/500 dilution (1.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Western blot - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)](https://www.abcam.com/ps/products/251/ab251145/Images/ab251145-405628-anti-atp6v1b1-antibody-epr16400-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)All lanes : Anti-ATP6V1B1 antibody [EPR16400] (ab192612) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDaThis data was developed using ab192612, the same antibody clone in a different buffer formulation.
-
![Western blot - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)](https://www.abcam.com/ps/products/251/ab251145/Images/ab251145-403031-anti-atp6v1b1-antibody-epr16400-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145) -
![Western blot - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)](https://www.abcam.com/ps/products/251/ab251145/Images/ab251145-399130-anti-atp6v1b1-antibody-epr16400-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)Anti-ATP6V1B1 antibody [EPR16400] (ab192612) at 1/10000 dilution + Human fetal kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDaThis data was developed using ab192612, the same antibody clone in a different buffer formulation.
-
![Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)](https://www.abcam.com/ps/products/251/ab251145/Images/ab251145-6-benefits-of-recombinant-antibodies.png)
Anti-ATP6V1B1 antibody [EPR16400] - BSA and Azide free (ab251145)