Anti-ATG4B antibody (ab72878)
Key features and details
- Rabbit polyclonal to ATG4B
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATG4B antibody
See all ATG4B primary antibodies -
Description
Rabbit polyclonal to ATG4B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human ATG4B aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab98899) -
Positive control
- This antibody gave a positive signal in Human Heart and Liver Tissue Lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).Target
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Function
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. -
Tissue specificity
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. -
Sequence similarities
Belongs to the peptidase C54 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23192 Human
- Entrez Gene: 66615 Mouse
- Omim: 611338 Human
- SwissProt: Q9Y4P1 Human
- SwissProt: Q8BGE6 Mouse
- Unigene: 283610 Human
- Unigene: 29087 Mouse
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Alternative names
- APG4 autophagy 4 homolog B antibody
- APG4B antibody
- ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
see all
Images
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Western blot - Anti-ATG4B antibody (ab72878)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: ATG4B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab72878 observed at 44 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab72878 was shown to recognize ATG4B when ATG4B knockout samples were used, along with additional cross-reactive bands. Wild-type and ATG4B knockout samples were subjected to SDS-PAGE. ab72878 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-ATG4B antibody (ab72878)All lanes : Anti-ATG4B antibody (ab72878) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72878 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ATG4B antibody (ab72878)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: ATG4B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab72878 observed at 44 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab72878 was shown to recognize ATG4B when ATG4B knockout samples were used, along with additional cross-reactive bands. Wild-type and ATG4B knockout samples were subjected to SDS-PAGE. ab72878 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-ATG4B antibody (ab72878)All lanes : Anti-ATG4B antibody (ab72878) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutes
