Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Ask1S83-G4] to ASK1 (phospho S83)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ASK1 (phospho S83) antibody [Ask1S83-G4]
    See all ASK1 primary antibodies

  • Description

    Rabbit monoclonal [Ask1S83-G4] to ASK1 (phospho S83)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ASK1 (phospho S83). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q99683

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: C6 (treated with pervanadate) cell extract; L929 (treated with 20% FBS) cell extract. Flow cyt: C6 cells (treated with pervanadate); K562 cells(treated with IFNa + IL-4 + pervanadate).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    Ask1S83-G4

  • Isotype

    IgG

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278547 at 1ng/mL.
  • Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547) at 0.01 µg/ml + L929 cell extract treated with 20% FBS

    Predicted band size: 155 kDa

  • Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with IFNa + IL-4 + pervanadate (green) using ab278547 at 5ng/mL.
  • Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or imatinib-treated (red) or pervandadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278547 at 1ng/mL.
  • Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    All lanes : Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547) at 0.01 µg/ml

    Lane 1 : C6 cell extract treated with imatinib
    Lane 2 : C6 cell extract treated with PV

    Predicted band size: 155 kDa

  • Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)
    Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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