Anti-ARPC1B antibody (ab99314)
Key features and details
- Rabbit polyclonal to ARPC1B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ARPC1B antibody
See all ARPC1B primary antibodies -
Description
Rabbit polyclonal to ARPC1B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human ARPC1B aa 272-332.
Database link: NP_005711.1 -
Positive control
- HeLa, 293T and NIH3T3 cell lysates
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General notes
This product was previously labelled as ARP2/3 subunit 1B
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab99314 was affinity purified using an epitope specific to ARPC1B immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ARPC1B antibody (ab99314)
ab99314 at 1 µg/ml detecting ARPC1B in HeLa whole cell lysate by WB following IP.
Lane 1: ab99314 at 3µg/mg of lysate.
Lane 2: IP with an antibody which recognizes an downstream epitope of ARPC1B.
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds. -
Western blot - Anti-ARPC1B antibody (ab99314)All lanes : Anti-ARPC1B antibody (ab99314) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : NIH3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 3 minutes
