Anti-APOBEC4 antibody (ab103737)
Key features and details
- Rabbit polyclonal to APOBEC4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-APOBEC4 antibody
See all APOBEC4 primary antibodies -
Description
Rabbit polyclonal to APOBEC4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human APOBEC4 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab119179) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Testis; Mouse Testis; Rat Testis; Human Fetal Brain; Human Fetal Lung.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications HorseDogPigApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).Target
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Function
Putative C to U editing enzyme whose physiological substrate is not yet known. -
Tissue specificity
Predominantly expressed in testis. -
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family. - Information by UniProt
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Database links
- Entrez Gene: 403314 Human
- Entrez Gene: 71281 Mouse
- Entrez Gene: 498251 Rat
- Omim: 609908 Human
- SwissProt: Q8WW27 Human
- SwissProt: Q497M3 Mouse
- SwissProt: Q6AXX9 Rat
- Unigene: 97335 Human
see all -
Alternative names
- ABEC4_HUMAN antibody
- APOBEC 4 antibody
- Apobec4 antibody
see all
Images
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Western blot - Anti-APOBEC4 antibody (ab103737)All lanes : Anti-APOBEC4 antibody (ab103737) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 : Testis (Rat) Tissue Lysate
Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)
Lane 5 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References (0)
ab103737 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-APOBEC4 antibody (ab103737)All lanes : Anti-APOBEC4 antibody (ab103737) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 : Testis (Rat) Tissue Lysate
Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)
Lane 5 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
