Anti-AMF antibody (ab226203)
Key features and details
- Rabbit polyclonal to AMF
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AMF antibody
See all AMF primary antibodies -
Description
Rabbit polyclonal to AMF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human AMF aa 508-558. The exact sequence is proprietary. NP_000166.2
Database link: P06744 -
Positive control
- IHC-P: Human ovarian carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate.
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General notes
This product was previously labelled as Glucose 6 phosphate isomerase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMF antibody (ab226203)All lanes : Anti-AMF antibody (ab226203) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody (ab226203)Formalin fixed, paraffin embedded human ovarian carcinoma tissue stained for AFM with ab226203 (1/5,000 dilution) in immunohistochemical analysis. DAB detection. Counterstain: hematoxylin (blue).
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Immunoprecipitation - Anti-AMF antibody (ab226203)AFM was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226203 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226203 at 1 µg/ml.
Lane 1: ab226203 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.