Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-AMCase antibody [EPR19985] - BSA and Azide free
See all AMCase primary antibodies
Description

Rabbit monoclonal [EPR19985] to AMCase - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Fr, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251464 is the carrier-free version of ab207168. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251464 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR19985
Isotype

IgG
Research areas

Western blot - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

All lanes : Anti-AMCase antibody [EPR19985] (ab207168) at 1/1000 dilution

Lane 1 : Rat stomach lysate
Lane 2 : Rat liver lysate
Lane 3 : Mouse stomach lysate
Lane 4 : Mouse liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

This data was developed using ab207168, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-2: 3 minutes; Lanes 3-4: 1 second.

CHIA mRNA is predominantly found in stomach, but not found in liver (PMID: 11085997; 12971947)

Negative control: Mouse and rat liver.
Western blot - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

Anti-AMCase antibody [EPR19985] (ab207168) at 1/1000 dilution + Mouse salivary gland lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

This data was developed using ab207168, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

CHIA mRNA is predominantly found in salivary gland.

PMID: 11085997 and 12971947.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

This data was developed using ab207168, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on chief cells of mouse gastric glands is observed [PMID:12971947]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on alveolar and airway epithelial cells of mouse lung is observed [PMID:16556727]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on chief cells of rat gastric glands is observed [PMID:12971947]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on airway epithelial cells of rat lung is observed [PMID:16556727]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative control: no staining on mouse liver [PMID: 15923370]. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse stomach tissue labeling AMCase with ab207168 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on chief cells of mouse gastric glands is observed [PMID: 12971947]. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)