Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19985] to AMCase - BSA and Azide free
  • Suitable for: WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-AMCase antibody [EPR19985] - BSA and Azide free
    See all AMCase primary antibodies

  • Description

    Rabbit monoclonal [EPR19985] to AMCase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251464 is the carrier-free version of ab207168. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251464 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Western blot - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    All lanes : Anti-AMCase antibody [EPR19985] (ab207168) at 1/1000 dilution

    Lane 1 : Rat stomach lysate
    Lane 2 : Rat liver lysate
    Lane 3 : Mouse stomach lysate
    Lane 4 : Mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab207168, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 3 minutes; Lanes 3-4: 1 second.

    CHIA mRNA is predominantly found in stomach, but not found in liver (PMID: 11085997; 12971947)

    Negative control: Mouse and rat liver.

  • Western blot - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Western blot - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Anti-AMCase antibody [EPR19985] (ab207168) at 1/1000 dilution + Mouse salivary gland lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab207168, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    CHIA mRNA is predominantly found in salivary gland.

    PMID: 11085997 and 12971947.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

    This data was developed using ab207168, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on chief cells of mouse gastric glands is observed [PMID:12971947]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on alveolar and airway epithelial cells of mouse lung is observed [PMID:16556727]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on chief cells of rat gastric glands is observed [PMID:12971947]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on airway epithelial cells of rat lung is observed [PMID:16556727]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling AMCase with ab207168 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative control: no staining on mouse liver [PMID: 15923370]. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Frozen sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Immunohistochemistry (Frozen sections) - Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    This data was developed using ab207168, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse stomach tissue labeling AMCase with ab207168 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on chief cells of mouse gastric glands is observed [PMID: 12971947]. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)
    Anti-AMCase antibody [EPR19985] - BSA and Azide free (ab251464)

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