Anti-MD2 antibody (ab24182)
Key features and details
- Rabbit polyclonal to MD2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MD2 antibody
See all MD2 primary antibodies -
Description
Rabbit polyclonal to MD2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatHumanIHC-P RatHumanWB MouseRatHuman
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Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MD2 antibody (ab24182) This image is courtesy of an Abreview submitted by C. GuerriAll lanes : Anti-MD2 antibody (ab24182) at 2 µg/ml (in 5% milk in TBS/T 0.1% (blocking buffer) for 12 hours at 4°C)
Lane 1 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours
Lane 2 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 3 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lane 4 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours
Lane 5 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 6 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocking Step: 5% Milk for 1 hour at room temperature. -
Western blot - Anti-MD2 antibody (ab24182)Anti-MD2 antibody (ab24182) at 1 µg/ml + Daudi cell lysate at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa
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Western blot - Anti-MD2 antibody (ab24182) -
Western blot - Anti-MD2 antibody (ab24182)All lanes : Anti-MD2 antibody (ab24182) at 1 µg/ml
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse testis lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa
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Western blot - Anti-MD2 antibody (ab24182)All lanes : Anti-MD2 antibody (ab24182)
All lanes : Rat spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa
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Western blot - Anti-MD2 antibody (ab24182)Anti-MD2 antibody (ab24182) at 1 µg/ml (mouse spleen lysate)
Predicted band size: 18 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD2 antibody (ab24182)ab24182 at 2µg/ml staining of MD2 in rat spleen cells by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-MD2 antibody (ab24182)Immunofluorescent analysis of MD2 in Rat Spleen cells with ab24182 at 10 μg/mL in ICC/IF.
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Immunocytochemistry/ Immunofluorescence - Anti-MD2 antibody (ab24182)Immunofluorescent analysis of MD2 in human spleen cells with ab24182 at 20 μg/mL in ICC/IF. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD2 antibody (ab24182)Immunohistochemical analysis of human spleen tissue stained for MD2 using ab24182 at 2.5μg/mL.