Anti-Aly/Ref antibody (ab101979)
Key features and details
- Rabbit polyclonal to Aly/Ref
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Aly/Ref antibody
See all Aly/Ref primary antibodies -
Description
Rabbit polyclonal to Aly/Ref -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Aly/Ref aa 25-75. (AAH52302.1)
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Positive control
- 293T and HeLa whole cell lysates
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General notes
This product was previously labelled as Aly
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Aly/Ref antibody (ab101979)All lanes : Anti-Aly/Ref antibody (ab101979) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aly/Ref antibody (ab101979)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling Aly/Ref with ab101979 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Aly/Ref antibody (ab101979)Detection of Aly/Ref by Western Blot of Immunprecipitate.
ab101979, at 1 µg/ml, staining Aly/Ref in 293T whole cell lysate immunoprecipitated using ab101979 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.
