Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789)
Key features and details
- Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Alkaline Phosphatase, Tissue Non-Specific antibody
See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies -
Description
Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Alkaline Phosphatase, Tissue Non-Specific aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab107105) -
Positive control
- This antibody gave a positive signal in Human kidney tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Target
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Function
This isozyme may play a role in skeletal mineralization. -
Involvement in disease
Hypophosphatasia
Hypophosphatasia childhood type
Hypophosphatasia infantile type -
Sequence similarities
Belongs to the alkaline phosphatase family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 249 Human
- Omim: 171760 Human
- SwissProt: P05186 Human
- Unigene: 75431 Human
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Alternative names
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
see all
Images
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Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789)Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
References (0)
ab86789 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789)Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
