Anti-AIF antibody - Mitochondrial Marker (ab1998)
Key features and details
- Rabbit polyclonal to AIF - Mitochondrial Marker
- Suitable for: WB, IHC-P, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AIF antibody - Mitochondrial Marker
See all AIF primary antibodies -
Description
Rabbit polyclonal to AIF - Mitochondrial Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human AIF aa 500-600.
Database link: O95831-1 -
Positive control
- WB: Human Caco-2, Daudi, HeLa, HepG2, MCF7, NIH3T3, YB2/0 and K562 cell lysate. Rat and mouse heart lysate. IHC-P: Human retina. ICC: Human U2OS and HeLa cells.
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General notes
Apoptosis Inducing FactorThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
AIF Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AIF antibody - Mitochondrial Marker (ab1998)All lanes : Anti-AIF antibody - Mitochondrial Marker (ab1998) at 1 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Mouse heart tissue lysate
Predicted band size: 67 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody - Mitochondrial Marker (ab1998)Immunohistochemistry of AIF in human retina with anti-AIF (IN) at 10 µg/ml. -
Western blot - Anti-AIF antibody - Mitochondrial Marker (ab1998)All lanes : Anti-AIF antibody - Mitochondrial Marker (ab1998) at 1 µg/ml
Lane 1 : Caco-2 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 67 kDa
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Western blot - Anti-AIF antibody - Mitochondrial Marker (ab1998)All lanes : Anti-AIF antibody - Mitochondrial Marker (ab1998) at 1 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : YB2/0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 67 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody - Mitochondrial Marker (ab1998) Image from Varecha M et al, J Biomed Sci. 2009 Jul 6;16:59, fig 2.ab1998 staining AIF in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
Samples were fixed using 4% paraformaldehyde.
Left image shows fixed cells labeled with ab1998 (red) and cyclophilin A (green).
Right image shows U2OS cell 6 hours after induction of apoptosis by 200 nM staurosporine.Note translocated of AIF to the nucleus upon induction of apoptosis. -
Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody - Mitochondrial Marker (ab1998)ICC/IF image of ab1998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1998, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
