Human Interferon gamma + IL5 FLUOROSPOT Kit (ab48431)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Fluorescent
- Sample type: Suspension cells
- Reacts with: Human
Overview
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Product name
Human Interferon gamma + IL5 FLUOROSPOT Kit -
Detection method
Fluorescent -
Sample type
Suspension cells -
Assay type
Sandwich (qualitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.
Diaclone Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
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Tested applications
Suitable for: FluroSpotmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 96 PVDF-bottomed-well plates. 5 units 10 units Anti-FITC antibody green fluorescence conjugate. 1 vial 2 vials Biotinylated detection antibody for IL-5 (resuspend in 0.55mL). 1 vial 2 vials Bovine Serum Albumin 1 x 1g 2 x 1g Human IFN Gamma Capture antibody 1 x 500µl 2 x 500µl Capture antibody for IL-5 1 x 500µl 2 x 500µl FITC conjugated detection antibody for IFNg (resuspend in 0.55mL) 1 vial 2 vials Streptavidin-phycoerythrin conjugate. 1 vial 2 vials -
Research areas
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Relevance
Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2. Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology. -
Cellular localization
Secreted -
Alternative names
- B cell differentiation Factor 1
- EDF
- Eosinophil differentiation factor
see all -
Database links
- Entrez Gene: 3458 Human
- Entrez Gene: 3567 Human
- SwissProt: P05113 Human
- SwissProt: P01579 Human
