Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AIF antibody [7F7AB10] (ab110327)

Key features and details

  • Mouse monoclonal [7F7AB10] to AIF
  • Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-AIF antibody [7F7AB10]
    See all AIF primary antibodies

  • Description

    Mouse monoclonal [7F7AB10] to AIF

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart; HeLa and HL60 cells. FFPE human heart tissue sections.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

     

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-AIF antibody [7F7AB10] (ab110327)
    Western blot - Anti-AIF antibody [7F7AB10] (ab110327)
    Anti-AIF antibody [7F7AB10] (ab110327) at 5 µg/ml + Isolated mitochondria from Human heart at 5 µg

    Predicted band size: 67 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody [7F7AB10] (ab110327)
    Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody [7F7AB10] (ab110327)
    Immunocytochemistry analysis using ab110327 at 5µg/ml staining AIF in HeLa cells (4% paraformaldehyde fixed and Triton X-100 permeabilized). The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • Flow Cytometry - Anti-AIF antibody [7F7AB10] (ab110327)
    Flow Cytometry - Anti-AIF antibody [7F7AB10] (ab110327)
    Flow cytometric analysis using ab110327 at 1µg/ml staining AIF in HL60 cells (blue). Isotype control antibody (red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody [7F7AB10] (ab110327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody [7F7AB10] (ab110327)
    IHC image of AIF staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110327, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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