Anti-AIF antibody (ab2086)
Key features and details
- Rabbit polyclonal to AIF
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AIF antibody
See all AIF primary antibodies -
Description
Rabbit polyclonal to AIF -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
KDGEQHEDLNEVAK
, corresponding to amino acids 593 - 606 of Human AIF. -
Positive control
- WB: CaCo-2, Daudi, HeLa, K562, NIH3T3 and YB2/0 cell lysate. ICC: K562 cells
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General notes
Apoptosis Inducing Factor.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AIF antibody (ab2086)All lanes : Anti-AIF antibody (ab2086) at 2 µg/ml
Lane 1 : CACO-2 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 67 kDa
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Immunocytochemistry - Anti-AIF antibody (ab2086)Immunocytochemical analysis of K562 cells with ab2086 at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.
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Western blot - Anti-AIF antibody (ab2086)All lanes : Anti-AIF antibody (ab2086) at 2 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : YB2/0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 67 kDa
