Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-AICL antibody [EPR22061] (ab221158)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22061] to AICL
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Human

Product name

Anti-AICL antibody [EPR22061]
See all AICL primary antibodies
Description

Rabbit monoclonal [EPR22061] to AICL
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U937 whole cell lysate. IHC-P: Human spleen and non-Hodgkin’s lymphoma tissues. ICC/IF: U937 cells. IP: U937 whole cell lysate.
General notes
This product was previously labelled as CLEC2B

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22061
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] (ab221158)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling AICL with ab221158 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Positive staining on human spleen (PMID: 9038101; PMID: 23929856). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-AICL antibody [EPR22061] (ab221158)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U937 (human histiocytic lymphoma cell line) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling AICL with ab221158 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic and membranous staining in U937 cell line.

Negative control: HeLa (PMID: 23929856).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] (ab221158)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin’s lymphoma tissue labeling AICL with ab221158 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Positive staining on human non-Hodgkin’s lymphoma (PMID: 23929856). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunoprecipitation - Anti-AICL antibody [EPR22061] (ab221158)

AICL was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) whole cell lysate with ab221158 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221158 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: U937 whole cell lysate 10 µg (Input).

Lane 2: ab221158 IP in U937 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221158 in U937 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.

The expression profile observed is due to glycosylation as described in the literature (PMID: 23929856).
Western blot - Anti-AICL antibody [EPR22061] (ab221158)

All lanes : Anti-AICL antibody [EPR22061] (ab221158) at 1/1000 dilution

Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 17 kDa
Observed band size: 26-28,45 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is due to glycosylation as described in the literature (PMID: 23929856).

Negative control: HeLa (PMID 23929856).
Anti-AICL antibody [EPR22061] (ab221158)

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