Anti-AICDA antibody - ChIP Grade (ab5197)
Key features and details
- Rabbit polyclonal to AICDA - ChIP Grade
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AICDA antibody - ChIP Grade
See all AICDA primary antibodies -
Description
Rabbit polyclonal to AICDA - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human AICDA aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab13718) -
Positive control
- This antibody gave a positive signal in AICDA 293T Cell Transient Overexpression Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718).Target
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Function
RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. -
Tissue specificity
Strongly expressed in lymph nodes and tonsils. -
Involvement in disease
Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers. -
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family. - Information by UniProt
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Database links
- Entrez Gene: 57379 Human
- Entrez Gene: 11628 Mouse
- Omim: 605257 Human
- SwissProt: Q9GZX7 Human
- SwissProt: Q9WVE0 Mouse
- Unigene: 149342 Human
- Unigene: 391503 Mouse
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Alternative names
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-induced cytidine deaminase antibody
see all
Images
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Western blot - Anti-AICDA antibody - ChIP Grade (ab5197)Anti-AICDA antibody - ChIP Grade (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Datasheets and documents
References (2)
ab5197 has been referenced in 2 publications.
- Dayal S et al. High resolution analysis of the chromatin landscape of the IgE switch region in human B cells. PLoS One 6:e24571 (2011). ChIP ; Human . PubMed: 21949728
- Duquette ML et al. AID binds to transcription-induced structures in c-MYC that map to regions associated with translocation and hypermutation. Oncogene 24:5791-8 (2005). PubMed: 15940261
Images
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Western blot - Anti-AICDA antibody - ChIP Grade (ab5197)Anti-AICDA antibody - ChIP Grade (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
