Anti-AGRP antibody [EPR18155-110] (ab254558)
![Anti-AGRP antibody [EPR18155-110] (ab254558)](https://www.abcam.com/ps/products/254/ab254558/Images/ab254558-3-anti-agrp-antibody-epr18155-110-1-immunohistochemistry-frozen-hypothalamus-mouse.jpg "Anti-AGRP antibody [EPR18155-110] (ab254558)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18155-110] to AGRP
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse
Overview
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Product name
Anti-AGRP antibody [EPR18155-110]
See all AGRP primary antibodies -
Description
Rabbit monoclonal [EPR18155-110] to AGRP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse hypothalamus and adrenal gland tissue. IHC-Fr: Mouse hypothalamus tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18155-110 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-AGRP antibody [EPR18155-110] (ab254558)
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse hypothalamus tissue labeling ab254558 with AGRP at 1/500 (1.12 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Specific staining on arcuate hypothalamic nucleus (PMID: 23638108) is observed.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGRP antibody (ab254558)Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling AGRP with ab254558 at 1/100 dilution (5.58 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on paraventricular nucleus of mouse hypothalamus (PMID: 10433222; 9311920) is observed. The section was incubated with ab254558 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGRP antibody (ab254558)Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling AGRP with ab254558 at 1/100 dilution (5.58 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on medullary cells of mouse adrenal gland (PMID: 28531318; 9311920) is observed. The section was incubated with ab254558 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .
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![Anti-AGRP antibody [EPR18155-110] (ab254558)](https://www.abcam.com/ps/products/254/ab254558/Images/ab254558-4-benefits-of-recombinant-antibodies.png)
Anti-AGRP antibody [EPR18155-110] (ab254558)
